PLATE WORKSPACE
Preparing image…
Connecting to shared storage…
Sign in with ChatGPTChoose whole plate only after checking every marker and looking for missing colonies.
SHARED VALIDATION DATA
Each submission is kept separately, so disagreements stay visible. Saved feedback is not automatically treated as ground truth or used to retrain the counter.
Sign in to view shared reviews.
FROM PHOTO TO REVIEWED COUNT
A first count you can inspect, one colony at a time. The app finds visible round centers, including those inside touching groups. You check the result and make the final corrections.
The warm-colored agar helps locate an oval counting area. An inset leaves out the plastic rim and some handwriting. Choose Set dish boundary, drag inside the outline to move it, or drag a square handle to resize it. Recount to apply.
Connected regions crossing the boundary are excluded. Check the dotted outline first.
The app compensates for uneven lighting and looks for spots darker than their surroundings. Size and shape checks filter out tiny noise, likely ink, and some implausible objects.
This version is designed for dark colonies on pale agar, like the example plates.
Each rounded lobe can have its own center, even when it touches another colony. The app places one marker per accepted center and adds them up.
There is no fixed limit of two or three colonies per touching group.
WHY TOUCHING COLONIES CAN STILL COUNT
Imagine turning a colony shape into a landscape. Its edges are low; points deeper inside are higher. Each round lobe tends to form a hilltop, with a dip at the join.
The app looks for distinct peaks in that landscape. A shallow dip is harder to separate, which is where Split touching colonies helps. This is a map of distance from the edge—not the colony’s physical height.
THE HUMAN PART
Zoom in and look for both incorrect markers and missed colonies. Use Add, Remove, and Checked to review the result. Right-click and drag pans in every tool mode.
Amber flags highlight joins, faint spots, irregular shapes, and edge detections. They are review hints, not measured probabilities of correctness.
Recounting applies new settings and replaces manual corrections after confirmation.
Image processing runs in the background on your device. There is no trained AI model or GPU involved. An uploaded photo stays in your browser until you choose Save feedback.
Export a CSV, annotation JSON, or marked image. Unsaved corrections stay only in this tab.
Saving keeps the original photo, automatic detections, corrected centers, reviewer name, notes, and review scope. Separate submissions preserve disagreements.
These are candidate labels for future validation and training. Saving feedback does not automatically train or change the counter.
Use an original, full-resolution photo taken directly above one dish, with even lighting and minimal glare or condensation.
Fully fused growth may hide the original colony shapes. Expert review is still needed, and real-plate accuracy has not yet been measured against verified annotations.
built with love for our friends at Johns Hopkins